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Image Search Results
Journal: BioMed Research International
Article Title: Fabrication and In Vitro Evaluation of 3D Printed Porous Polyetherimide Scaffolds for Bone Tissue Engineering
doi: 10.1155/2019/2076138
Figure Lengend Snippet: Results of biocompatibility analysis for the 3D PEI scaffold and PEI slice. (a, b) Fluorescence images of nuclei of different samples. Scale: 200 μ m. (c) Live/dead cell staining. Scale: 100 μ m. (d) Cytotoxicity test. (e) SEM images of cell morphology. Scale: 20 μ m. (f) Cell proliferation. ∗ Statistically significant at p < 0.05 vs. the control.
Article Snippet: Then, the
Techniques: Fluorescence, Staining, Control
Journal: Virus research
Article Title: The neuropathological mechanism of EV-A71 infection attributes to inflammatory pryoptosis and viral replication via activating the hsa_circ_0045431/ hsa_miR_584/NLRP3 regulatory axis.
doi: 10.1016/j.virusres.2023.199195
Figure Lengend Snippet: Fig. 1. EV-A71 infection triggers cell death of SH-SY5Y cells. (A) The percentage of cells undergoing cell death was calculated through Annexin V-FITC/PI double staining. (B) The cell viability was conducted with Cell Counting Kit-8. (C) Levels of LDH released into the cell culture medium from membrane pores were examined using an LDH Cytotoxicity Assay Kit. (D) Caspase1 activity was assessed with a Caspase1 Activity Assay Kit. .
Article Snippet: For the colorimetric method, caspase1 activity was determined using a
Techniques: Infection, Double Staining, Cell Counting, Cell Culture, Membrane, LDH Cytotoxicity Assay, Activity Assay
Journal: Virus research
Article Title: The neuropathological mechanism of EV-A71 infection attributes to inflammatory pryoptosis and viral replication via activating the hsa_circ_0045431/ hsa_miR_584/NLRP3 regulatory axis.
doi: 10.1016/j.virusres.2023.199195
Figure Lengend Snippet: Fig. 2. EV-A71 infection induces NLRP3-dependent pyroptosis in SH-SY5Y cells. (A) NLRP3, ASC, Casepase1, Gasdermin D, IL-1β and IL-18 mRNA levels were analyzed by qRT-PCR. (B) The abundance of NLRP3, ASC, pro-Caspase1, cleaved Caspase 1, Gasdermin D, IL-1β and IL-18 was determined by WB analysis. (C) At 48 and 72 h post infection, infected SH-SY5Y cells were subjected to indirect immunofluorescence assays with anti-VP1 (red) and anti-Caspase-1 (green) antibodies. (D) Secretion of IL-1β and IL-18 upon infection with EV-A71 was measured with a commercial ELISA kit. .
Article Snippet: For the colorimetric method, caspase1 activity was determined using a
Techniques: Infection, Quantitative RT-PCR, Immunofluorescence, Enzyme-linked Immunosorbent Assay
Journal: Virus research
Article Title: The neuropathological mechanism of EV-A71 infection attributes to inflammatory pryoptosis and viral replication via activating the hsa_circ_0045431/ hsa_miR_584/NLRP3 regulatory axis.
doi: 10.1016/j.virusres.2023.199195
Figure Lengend Snippet: Fig. 8. has_circ_0045431/has-miR-584/NLRP3 regulatory axis promotes EV-A71-induced pyroptosis formation. (A) SH-SY5Y cells with different treatments were subjected to WB analysis for NLRP3, ASC, pro-Caspase1, cleaved Caspase 1, Gasdermin D, IL-1β and IL-18 expression. (B) The cell culture supernatant was subjected to the examination of ELISA for IL-1β and IL-18 concentration.
Article Snippet: For the colorimetric method, caspase1 activity was determined using a
Techniques: Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay, Concentration Assay